responsible trace mineral management
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Calculating trace mineral levels in the basal diet is required to feed dairy cows according to the NASEM 2021 guidelines

The key takeaway from this article

  • The NASEM 2021 guidelines for dairy cattle are based on total diet.
  • To feed dairy cows according to the NASEM 2021 guidelines, trace mineral levels in the basal diet ingredients should be taken into account
  • Recommendations based on values from 5,500 dairy diets can be used to reduce he risk of deficiencies to less than 1%, while avoiding over-feeding of trace minerals to dairy cows

How much trace minerals do cows need?

Trace mineral requirements of dairy cattle depend on genetic potential, milk production, reproductive status, days in milk and the composition of the ration. Nutritionists often have concerns about trace mineral deficiencies in cattle, particularly about copper deficiency in cows.

As a result, it is standard practice to supplement cattle with trace minerals at levels exceeding the NASEM 2021 guidelines. Over-feeding of trace minerals for cattle is a common problem1,2,3,4,5,6,7,8, increasing the risk of trace minerals toxicity in cattle and trace mineral soil contamination. This emphasizes the importance of aligning dietary mineral levels with the specific animal requirements to balance production efficiency and environmental sustainability.

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Calculating the optimal level of trace mineral supplementation with the Responsible Trace Mineral approach

How much trace minerals do cows need? Calculating the correct amount of trace minerals for cattle can be done in 3 easy steps.

Step 1: Understanding the NASEM 2021 recommendations for trace minerals for cattle

The National Academies of Sciences, Engineering, and Medicine 2021 guidelines3, formerly the National Research Council (NRC) guidelines9, provide scientifically validated recommendations for dairy nutrition, including trace mineral requirements. The NASEM 2021 guidelines are based on a comprehensive evaluation of all publicly available scientific evidence. The latest update of the NASEM 2021 guidelines reflects minor changes for trace mineral requirements, particularly for Cr, Cu, Mn and Zn, compared to previous NRC (2001) recommendations.

The NASEM 2021 guidelines serve as a benchmark for calculating how much trace minerals cows need and for formulating diets that meet the specific needs of lactating and non-lactating dairy cows. The NASEM 2021 recommendations are based on total diet. Main recommendations and differences between the NASEM 2021 guidelines and the 2001 NRC guidelines have been summarized in the Trouw Nutrition Technical Brochure entitled: “NRC 2001 vs NASEM 2021 comparison of trace mineral and vitamin requirements for dairy cattle”

Step 2: determine how much trace mineral is already present in the basal ration
The basal ration of dairy cows is defined as the combination of all forages and by-products, without compound feed and supplements. Neglecting the trace minerals naturally present in forages and by-products can result in over-supplementation of trace minerals to dairy cows.

The simplest way to account for trace mineral levels in the basal ration is by using the values from a model based on 5,000 dairy diets analysed by Trouw Nutrition R&D. This option is highly practical and balances the risk between over and under-supply using robust methodology. The method is based on modelling the risk for inadequate trace mineral intake. It makes use the boundaries for adequacy for trace minerals. Through the analysis of approximately 5,000 different diets from different farms, Trouw Nutrition's R&D team calculated the distribution of copper (Cu), zinc (Zn), manganese (Mn), and iron (Fe) levels across those 5,000 dairy diets10 (see Table 1).

Copper (Cu) Zinc (Zn) Manganese (Mn)
Percentile Level Percentile Level Percentile Level
Highest 36 Highest 148 Highest 204
99% 18 99% 66 99% 100
90% 12 90% 49 90% 64
50% 9 50% 37 50% 46
10% 7 10% 28 10% 34
1% 5 1% 21 1% 25
Lowest 4 Lowest 15 Lowest 18
Table 1, Trace mineral levels (ppm) for Cu, Zn, Mn and Fe in 5,000 different dairy rations: distribution across percentiles.
Trace element
Copper (ppm) Zinc (ppm) Manganese (ppm)
NASEM 2021 recommendation 10 66 37
1% percentile basal diet 5 21 25
Supplementation required 5 45 12
Trace element
Copper (ppm) Zinc (ppm) Manganese (ppm)
NASEM 2021 recommendation 10 66 37
1% percentile basal diet 5 21 25
Supplementation required 5 45 12

Table 2: Trace mineral content of the basal diet and NASEM 2021 guideline values to calculate how much trace mineral must be supplementation to dairy cattle to prevent trace mineral deficiency in cattle, while avoiding the risk of trace mineral toxicity in dairy cattle.

The 1% percentile from Table 1 is being used to calculate the minimum level of trace mineral that needs to be supplemented to minimise the risk of underfeeding. If the 1% percentile is being used, there is a 99% chance that the actual diet fed on the farm has a higher level of trace minerals compared to the value that is being used in the calculations, reducing the risk of trace mineral deficiency of dairy cattle to 1% or less.

The 99% percentile from Table 1 is being used to compare the maximum level of trace mineral that is supplemented to the tolerance level according to the NASEM 2021 guidelines, thus reducing the risk of overfeeding. If the 99% percentile is being used to calculate the risk of overfeeding, it means there is only a 1% chance that the actual diet used on the farm has a higher level of trace minerals compared to what was used in the calculation. This reduces the risk over overfeeding and trace mineral toxicity in cattle to 1% or less.

Table 2 provides calculations of supplementation levels required to avoid under-supply based on combining data from Table 1 on minimal basal diet trace mineral content with recommended values for trace mineral supplementation of cattle from the NASEM 2021 guidelines.

Trace element
Copper (ppm) Zinc (ppm) Manganese (ppm)
NASEM 2021 upper tolerance limit 25 130 250
99% percentile basal diet 18 69 100
Maximum supplementation to stay below tolerance limit 7 61 150
Trace element
Copper (ppm) Zinc (ppm) Manganese (ppm)
NASEM 2021 upper tolerance limit 25 130 250
99% percentile basal diet 18 69 100
Maximum supplementation to stay below tolerance limit 7 61 150

Table 3. Trace mineral content of the basal rations and maximum tolerance levels from the NASEM 2021 guidelines are used to calculate how much trace mineral can be supplementation if the risk of trace minerals toxicity in cattle needs to be minimised.

Table 3 compares the maximum amount of trace mineral that is likely to be supplied on top of the levels in the basal diets. It compares these values with tolerance levels according to the NASEM 2021 guidelines. The risk of feeding above tolerance levels is calculated based on combining data from Table 1 with data on maximum dietary tolerance level for trace minerals. According to NASEM 2021 guidelines, the upper tolerance limit is a level at which sub-clinical effects may occur. The level at which trace minerals toxicity occurs is much higher. For example, the NASEM 2021 guidelines suggest the upper tolerance level for manganese is 250 ppm, whereas the toxic level is 1,000 ppm.

Recommendations for Cu, Zn, Mn and Fe supplementation of dairy cattle

Recommendation for copper supplementation of dairy cattle
Risk of undersupply vs. oversupply: at the 1% percentile, the basal diet provides 5 ppm Cu, which partially meets the cow's requirement for copper. At the 99% percentile, the same cow would receive up to 18 ppm Cu from the basal diet. Considering the maximum tolerable limit of 25 ppm we would still be 7 ppm Cu under the maximum tolerable level, but care must be taken to avoid exceeding this threshold.

Copper supplementation needed: to avoid copper deficiency symptoms in cattle and to reach the minimum NASEM 2021 requirement of 10 ppm Cu, an additional 5 ppm Cu must be added. To avoid copper toxicity in cattle, supplementation should not exceed 7 ppm Cu, in order to stay below the upper tolerable limit of 25 ppm. Thus, adding 5-7 ppm Cu is sufficient to meet the NASEM 2021 recommendation and to avoid copper deficiency in cows, while avoiding over-feeding and copper toxicity in dairy cattle.

Recommendations for Zinc supplementation of dairy cattle
Risk of undersupply vs. oversupply: at the 1% percentile, the basal diet provides 21 ppm Zn, which partially meets the NASEM 2021 requirement for dairy cattle. At the 99% percentile, the same cow would receive up to 69 ppm Zn from the basal diet. Considering the maximum tolerable limit in the NASEM guidelines of 30 ppm Zn, we would still be 61 ppm Zn under the maximum tolerable level.

Zinc supplementation needed: to reach the minimum NASEM 2021 requirement of 66 ppm Zn, an additional 45 ppm Zn must be added. To avoid oversupply and trace mineral toxicity in cattle, supplementation should not exceed 61 ppm Zn to stay below the upper tolerable limit of 130 ppm in the NASEM 2021 guidelines. Thus, adding 45-61 ppm Zn is sufficient to meet the cow's needs while avoiding overfeeding.

Recommendations for Manganese supplementation of dairy cattle
Risk of undersupply vs. oversupply: at the 1% percentile, the basal diet provides 25 ppm Mn, which partially meets the NASEM 2021 recommendations for manganese supplementation of dairy cattle. At the 99% percentile, the same cow would receive up to 100 ppm Mn from the basal diet. Considering the maximum tolerable limit of 250 ppm Mn, we would still be 150 ppm Mn under the maximum tolerable level in the NASEM 2021 guidelines.

Manganese supplementation needed: to reach the minimum requirement in the NASEM 2021 guidelines of 37 ppm Mn, an additional 12 ppm Mn must be added. To avoid oversupply, supplementation should not exceed 150 ppm Mn to stay below the upper tolerable limit of 250 ppm in the NASEM 2021 guidelines. Thus, adding 12-150 ppm Mn is sufficient to meet the recommendations in the NASEM 2021 guidelines, while avoiding overfeeding and trace mineral toxicity in cattle.

It is not recommended to supplement Iron to dairy cows
Dietary iron level at the 1% percentile is 100 ppm, which already exceeds the NASEM 2021 recommendation of 20 ppm iron for lactating cows 5 times. Consequently, additional iron supplementation is unnecessary in adult dairy cattle diets and increases the risk of trace minerals toxicity in cattle.

Trace Mineral Total NASEM 2021 Requirement (ppm) Contribution from Basal Diet (ppm) Shortfall (ppm) Amount of required from Selko IntelliBond (mg/day) Amount of Selko IntelliBond required (mg/cow/day)
Cu 10 5 10 - 5 = 5 22.5 x 5 = 113 113 / 0.54 = 208
Zn 66 21 66 - 21 = 45 22.5 x 45 = 1,013 1,013 / 0.55 = 1,841
Mn 37 25 37 - 25 = 12 22.5 x 12 = 270 270 / 0.44 = 614
Trace Mineral Total NASEM 2021 Requirement (ppm) Contribution from Basal Diet (ppm) Shortfall (ppm) Amount of required from Selko IntelliBond (mg/day) Amount of Selko IntelliBond required (mg/cow/day)
Cu 10 5 10 - 5 = 5 22.5 x 5 = 113 113 / 0.54 = 208
Zn 66 21 66 - 21 = 45 22.5 x 45 = 1,013 1,013 / 0.55 = 1,841
Mn 37 25 37 - 25 = 12 22.5 x 12 = 270 270 / 0.44 = 614

Table 4: Trace mineral supplementation of dairy cows using Selko IntelliBond products. This table details the shortfall in trace mineral supply from the basal diet, the amount of supplementation required from Selko IntelliBond, and the resulting inclusion rates required to meet the NASEM 2021 guidelines for trace mineral supplementation of dairy cows.

Meeting the shortfall with Selko IntelliBond products

The final step is to calculate the total amount of Selko IntelliBond trace minerals that needs to be included in a diet of a cow consuming 22.5 kg of DM to meet the recommended NASEM 2021 levels for copper of 10 ppm, the NASEM 2021 recommended levels for zinc of 66 ppm and the NASEM 2021 recommended levels for manganese of 27 ppm. Table 4 shows the calculation of the shortfall, and the Selko IntelliBond inclusion rates required to balance the diet and to meet the NASEM 2021 guidelines for dairy cattle.

After supplementation with Selko IntelliBond hydroxy trace minerals, the final diet precisely meets the recommended trace mineral requirements from the NASEM 2021 guidelines, ensuring both nutritional adequacy and environmental stewardship:

  • Copper inclusion from Selko IntelliBond C: 5 ppm (resulting in Cu: 10 ppm in total diet)
  • Zinc inclusion from Selko IntelliBond Z: 45 ppm (resulting in Zn: 66 ppm in total diet)
  • Manganese inclusion from Selko IntelliBond M: 12 ppm (resulting in Mn: 37 ppm)

Figure 1: Relative bioavailability of hydroxy trace minerals in Selko IntelliBond compared to organic trace mineral sources and sulphates.

Considering relative bioavailability of trace mineral sources used for supplementation

The recommendations in the NASEM 2021 guidelines are based on trace minerals derived from inorganic sources, which are still widely used in livestock diets. Selko IntelliBond hydroxy trace minerals can have a significantly higher relative bioavailability compared to sulphates, due to:

  • Reduced interactions with vitamins and other trace metal ions in the feed matrix
  • Better stability in the rumen and less antagonism with other minerals in the gastro-intestinal tract
  • Improved absorption compared to sulphates and some traditional organic mineral sources.

    Comparative studies11,12,13,14,15 have consistently shown the superior bioavailability of Selko IntelliBond hydroxy trace minerals over sulphate-based minerals sources (see Figure 1). There is however variation between different studies. The relative bioavailability of the copper in Selko IntelliBond C compared to copper sulphate varies between 112% and 196%. This is related to the fact that trace mineral absorption in dairy cattle is a regulated process and bioavailability is different between sources but also depending on the level of trace mineral fed to the animal. If fed below requirements, absorption is high, if fed well above requirements, absorption is low. This reduces the risk of trace mineral deficiency in cattle for diets with low levels of trace minerals in the basal diet. If supplementation levels are being reduced, absorption is likely to be upregulated, reducing the risk of underfeeding of trace minerals to dairy cows.

Alternative ways to calculate the amount of trace minerals already present in the basal ration

Step 2 of the previous method to calculate the amount of trace mineral present in the basal ration was based on the “Boundaries of accuracy method”, which was based on trace mineral levels in 5,000 different rations.

Calculating the amount of trace minerals in a basal diet based on the values found in 5,000 diets is a robust and simple method. An alternative approach would be to calculate the amount of trace minerals in a basal diet based on analysis of forages and byproducts. This can be done by analysing the forages and byproducts used on the farm for which the diet is being formulated, or it can be based on typical values based on the analysis of a representative number of samples.

Using typical values trace mineral levels in forages and by-products for dairy diets
In most cases, testing is not feasible, and relying on typical mineral values from trusted sources can provide a practical baseline for supplementation. Typical values for trace mineral levels in forages and by-products can serve as a guideline. Table 5 provides approximate ranges of key trace minerals found in commonly used feed ingredients.

Feed Component Copper (Cu) Manganese (Mn) Zinc (Zn) Iron (Fe)
Alfalfa Hay 8–10 mg/kg DM 30–50 mg/kg DM 20–30 mg/kg DM 100–200 mg/kg DM
Barley Hay 4–6 mg/kg DM 25–35 mg/kg DM 20–30 mg/kg DM 60–110 mg/kg DM
Bermuda Grass 3–5 mg/kg DM 20–30 mg/kg DM 15–25 mg/kg DM 50–100 mg/kg DM
Canola Meal 10–15 mg/kg DM 30–50 mg/kg DM 40–60 mg/kg DM 80–120 mg/kg DM
Citrus Pulp 5–7 mg/kg DM 10–20 mg/kg DM 15–25 mg/kg DM 40–80 mg/kg DM
Corn (Grain) 2–4 mg/kg DM 5–10 mg/kg DM 15–25 mg/kg DM 20–50 mg/kg DM
Corn Silage 4–6 mg/kg DM 15–25 mg/kg DM 10–20 mg/kg DM 50–100 mg/kg DM
Cottonseed Hulls 3–5 mg/kg DM 10–15 mg/kg DM 5–10 mg/kg DM 30–60 mg/kg DM
Distillers Grains 5–10 mg/kg DM 30–60 mg/kg DM 50–80 mg/kg DM 80–120 mg/kg DM
Grass Hay 5–8 mg/kg DM 20–40 mg/kg DM 15–25 mg/kg DM 50–150 mg/kg DM
Oat (Forage) 6–8 mg/kg DM 25–35 mg/kg DM 20–25 mg/kg DM 60–120 mg/kg DM
Red Clover 7–9 mg/kg DM 25–45 mg/kg DM 25–35 mg/kg DM 80–130 mg/kg DM
Ryegrass 5–7 mg/kg DM 30–40 mg/kg DM 20–25 mg/kg DM 70–120 mg/kg DM
Sorghum Silage 4–6 mg/kg DM 15–25 mg/kg DM 10–20 mg/kg DM 50–100 mg/kg DM
Soybean Hulls 5–8 mg/kg DM 20–30 mg/kg DM 20–30 mg/kg DM 50–100 mg/kg DM
Soybean Meal 15–20 mg/kg DM 20–30 mg/kg DM 40–60 mg/kg DM 100–150 mg/kg DM
Wheat Straw 3–5 mg/kg DM 10–20 mg/kg DM 5–10 mg/kg DM 30–60 mg/kg DM
White Clover 6–8 mg/kg DM 20–40 mg/kg DM 20–30 mg/kg DM 70–120 mg/kg DM
Table 5: Trace mineral concentration in common ingredients used in cattle diets. The trace mineral levels in ingredients for dairy rations in the table are approximate values derived from scientific literature, feed composition tables (published by research institutes and organizations) and from private commercial forage and feed testing labs.
Using the selected ingredients and their typical mineral content, the contribution of each trace mineral (Zn, Mn, and Cu) from the basal diet can be calculated. **Table 6** provides an example of a breakdown of the mineral contribution from each ingredient on a dry matter basis.

Ingredient % of DM Zn Contribution (ppm) Mn Contribution (ppm) Cu Contribution (ppm)
Corn Silage 35 25×0.35=9 12×0.35=4 4×0.35=1.4
Grass Hay 20 35×0.20=7 30×0.20=6 6×0.20=1.2
Ground Corn 15 20×0.15=3 5×0.15=1 3×0.15=0.45
Soybean Meal 5 40×0.05=2 30×0.05=2 15×0.05=0.75
Total Contribution 21 ppm 13 ppm 4 ppm
Table 6: Example on how to calculate trace mineral contributions from basal diet ingredients. This table shows the percentage of each ingredient in the diet, the trace mineral content, and the resulting contribution for zinc, manganese, and copper on a ppm basis.
From the calculated values, the total trace mineral contribution of the base diet for a 22.5 kg DMI was determined. **Table 7** provides these values. The next steps in the calculation will be the same as the steps -after the contribution of the basal diet was calculated based on values from 5,000 diets.

Trace Mineral Contribution from Basal Diet (ppm) Contribution from Basal Diet (mg/day)
Zinc (Zn) 21 21×22.5=467
Manganese (Mn) 13 13×22.5=280
Copper (Cu) 4 4×22.5=86
Table 7: Example on how to calculate trace mineral contributions from basal diet ingredients. This table shows the percentage of each ingredient in the diet, the trace mineral content, and the resulting contribution for zinc, manganese, and copper on a ppm basis.

Calculating the correct level of trace mineral supplementation as part of a Responsible Trace Mineral approach

A crucial element of a Responsible Trace Mineral approach is determining the trace mineral requirements of dairy cattle. The recommended trace mineral supplementation levels in NASEM 2021 guidelines are based on total diet. Ignoring the contribution of the basal diet will lead to over-supply of trace minerals and will increase the risk of trace mineral toxicity in cattle. A robust, accurate and easy to apply method for calculating the trace mineral levels in the basal diet is based on values from 5,000 diets that have been analysed by Trouw Nutrition R&D.

If the total diet does not meet the herd's mineral requirements, supplementation should be provided to avoid copper deficiency in cows as well as deficiencies of zinc and manganese. Iron levels in the basal diet are already above the minimum recommended levels in the NASEM 2021 guidelines, therefore, supplementation of lactating dairy cows with iron will only increase the risk of iron toxicity and contamination of soil with iron.

If a cattle ration has mineral levels above the NASEM 2021 guidelines, reducing the levels of trace mineral supplements for cattle offers multiple benefits. Eliminating unnecessary mineral supplements leads to cost savings, improved performance of dairy cattle and to a significant reduction in mineral excretion into the environment.

By aligning trace mineral feeding with precision nutrition strategies, Selko IntelliBond empowers producers to achieve better herd performance, higher profitability, and a more sustainable future for dairy farming.

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References about calculating the correct level of trace mineral nutrition to meet the NASEM 2021 guidelines

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  2. Castillo, A.R., St-Pierre, N.R., Silva del Rio, N, and Weiss, W.P. (2013). Mineral concentrations in diets, and milk and their value in estimating on-farm excretion of manure minerals in lactating dairy cows. J. Dairy Sci. 96(5):3388-3398.
  3. National Academies of Sciences, Engineering, and Medicine (NASEM). (2021). Nutrient Requirements of Dairy Cattle: Eighth Revised Edition. Washington, DC: The National Academies Press. https://doi.org/10.17226/25806.
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  6. Counotte, G., Holzhauer, M., Carp-van Dijken, S., Muskens, J., & Van der Merwe, D. (2019). Levels of trace elements and potential toxic elements in bovine livers: A trend analysis from 2007 to 2018. PLOS ONE, 14(4), e0214584. https://doi.org/10.1371/journal.pone.0214584.
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  9. National Research Council (NRC). (2001). Nutrient Requirements of Dairy Cattle: Seventh Revised Edition, 2001. Washington, DC: The National Academies Press. https://doi.org/10.17226/9825.
  10. Daniel JB, Martin-Tereso, J. (2024). Modelling risk for inadequate trace mineral intake. Presented at: Trouw Nutrition's DairyNutriVision 2024; Rotterdam, The Netherlands. Data not published.
  11. Shaeffer, G.L, Lloyd, K.E, and J.W. Spears (2017). Bioavailability of zinc hydroxychloride relative to zinc sulphate in growing cattle fed a corn-cottonseed hull-based diet. Animal Feed Science and Technology 232 (2017) 1–5.
  12. Spears, W, Kegley, E.B. and L.A. Mullis (2004) Bioavailability of copper from tribasic copper chloride and copper sulphate in growing cattle. Animal Feed Science and Technology 116: 1–13.
  13. VanValin, K. R., Genther-Schroeder, O.N., Laudert, S.B. and S. L. Hansen (2019). Relative bioavailability of organic and hydroxy copper sources in growing steers fed a high antagonist diet. J. Anim. Sci. 97:1375-1383.
  14. Caldera, C.E, Weigel, B, Kucharczyk, V.N, Sellins, K.S, Archibeque, S.L, Wagner, J.J, Han, H, Spears, J.B. and T.E. Engle (2019). Trace mineral source influences ruminal distribution of copper and zinc and their binding strength to ruminal digesta. J. Anim. Sci., 97:1852-1864.
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