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Rumen protected calcium gluconate increases volatile fatty acid levels in the hindgut of beef cattle

Key take-away messages

  • Selko LactiBute contains rumen protected calcium gluconate which has a prebiotic effect in the hindgut of beef cattle
  • Feeding Selko LactiBute to steers increases volatile fatty acids levels in the hindgut
  • Increased levels of butyrate in the hindgut of beef cattle can improve hindgut health and reduce the risk of "leaky gut"

Managing hindgut acidosis of beef cattle with rumen protected calcium gluconate

Intestinal health of beef feedlot cattle is essential for optimal growth, feed efficiency and productivity. While the focus often rests on the rumen, the hindgut plays a critical role in the digestive processes and general health of beef cattle. Hindgut fermentation of excessive carbohydrate that are typical for beef feedlot diets can result in hindgut acidosis. Hindgut acidosis in beef cattle can have a negative impact on gastrointestinal microbiota and on gut barrier function, leading to a phenomenon called “leaky gut”[1] . As a result of leaky gut, bacterial endotoxins can reach the blood circulation. This causes systemic inflammation and activation of the immune system[2] , with a significant energy cost for the animal. Systemic immune activation can alos cause a reduction of dry matter intake[3] , leading to a further negative impact on growth performance of beef cattle.

Risk factors for rumen acidosis of beef cattle also increase the risk for hindgut acidosis. Signs commonly associated with rumen acidosis of cattle, such as loose and frothy faeces with mucin casts, are also common in beef cattle with hindgut acidosis[4] .

Nutritional interventions aimed at reducing the risk of hindgut acidosis in dairy cows should target the hindgut[5] . Trials with Selko LactiBute, containing rumen protected calcium gluconate have shown to improve gut barrier function, to change the volatile fatty acid profile in faeces and to increase milk production of dairy cows[6,7,8,9]

Effect of rumen protected calcium gluconate on hindgut VFA profile of beef cattle

Reducing hindgut acidosis in beef cattle through nutritional interventions should target the hindgut[5] . Trials with Selko LactiBute have shown that rumen protected calcium gluconate can improve gut barrier function[6] , and increase feed efficiency and average daily gain of beef cattle[7,8] . The impact of feeding Selko LactiBute on volatile fatty acid levels in the hindgut of beef steers was investigated in a trial carried out by Dr. Todd Callaway at the University of Georgia[9] .

Material and methods

Two groups of 10 steers each were included in the trial. Age of the animals at the start of the trial was around 13 months, initial bodyweight was 449 +/- 10 kg. Both groups of steers were on a diet of 75% rolled corn, 10% corn gluten feed, 7% soyhull pellets, 6% soybean meal and 0.5% urea. After an adaptation period of 3 days, one of the 2 groups was fed Selko LactiBute at a dose of 16 grams/animal/day for a period of 7 days. The other group served as controls. At slaughter, tissue samples and digesta samples of rumen, duodenum, ileum, cecum, and colon were taken. Volatile fatty acid levels were measured and the microbiome was determined.

Results, microbiome of the hindgut

A clear shift towards butyrate producing bacteria was seen in the ceacum and colon.

Gut section
Rumen Duodenum Ileum Cecum Colon Rectum
Acetate LAC 56.17 3.32 29 79.7a 56.4 52.7a
CON 57.63 2.29 24.3 61.8b 35.6 32.0b
P-val 0.88 0.24 0.69 0.01 0.08 0.02
Propionate LAC 41.5 -- 8.86 28.79a 21.2a 18.6a
CON 42.3 0.06 4.96 17.86b 11.3b 9.09b
P-val 0.90 0.19 0.43 0.02 0.05 0.01
Butyrate LAC 8.8 -- 3.59 14.1 9.13 6.82
CON 10.68 -- 4.51 13.1 4.9 6.85
P-val 0.55 -- 0.70 0.78 0.10 0.99
Isobutyrate LAC 0.98 -- 0.12 0.61 0.40 0.18
CON 1.02 -- 0.13 0.69 0.37 0.2
P-val 0.91 -- 0.88 0.38 0.90 0.85
Gut section
Rumen Duodenum Ileum Cecum Colon Rectum
Acetate LAC 56.17 3.32 29 79.7a 56.4 52.7a
CON 57.63 2.29 24.3 61.8b 35.6 32.0b
P-val 0.88 0.24 0.69 0.01 0.08 0.02
Propionate LAC 41.5 -- 8.86 28.79a 21.2a 18.6a
CON 42.3 0.06 4.96 17.86b 11.3b 9.09b
P-val 0.90 0.19 0.43 0.02 0.05 0.01
Butyrate LAC 8.8 -- 3.59 14.1 9.13 6.82
CON 10.68 -- 4.51 13.1 4.9 6.85
P-val 0.55 -- 0.70 0.78 0.10 0.99
Isobutyrate LAC 0.98 -- 0.12 0.61 0.40 0.18
CON 1.02 -- 0.13 0.69 0.37 0.2
P-val 0.91 -- 0.88 0.38 0.90 0.85

Table 1: Levels of acetate, propionate, butyrate and isobutyrate in rumen, duodenum, ileum, caecum, colon and rectum of steers fed Selko LactiBute containing rumen protected calcium gluconate vs. controls.Volatile fatty acid values in bold are statistically significant.

Results, volatile fatty acid profile in the hindgut

Volatile fatty acid (VFA) profiles are presented in Table 1. VFA profiles in rumen, duodenum and ileum did not change, but adding Selko LactiBute to the diet resulted in an increase of acetate and propionate levels in ceacum, colon and rectum. Butyrate levels tended to be higher in the colon (p= 0.10).

Conclusion

These results indicated that rumen protected calcium gluconate has a prebiotic effect in the hindgut of beef cattle and can be used to manipulate the microbial population to increase VFA production in the hindgut of beef cattle.

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Selko LactiBute, Feeding beef to maximise efficiency of beef cattle operations

Improving feed efficiency and performance is a crucial goal for all beef producers. With rising costs and growing pressure to achieve more with less, finding effective solutions is more important than ever. Enhancing gut health of beef cattle has emerged as a key strategy for significantly improving diet utilization. Although historically overlooked, recent research reveals that targeted supplementation at the hindgut level can yield remarkable results. To discover how you can make substantial gains in your beef production, download our comprehensive brochure today!

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References about managing hindgut health of receiving beef cattle

  1. Horst, E.A, Mayorga, E.J, Rodriguez-Jimenez, S, Abeyta, M.A, Goetz, B.M, Carta, S, Al-Qaisi, M, Kvidera, S.K. and L. H. Baumgard (2019). Causes and Metabolic Consequences of Leaky Gut. 2019 Cornell Nutrition Conference Proceedings.
  2. Trevisi E., Massimo A., Cogrossi S., Bertoni G (2011). Metabolic stress and inflammatory response in high-yielding, periparturient dairy cows. Research in Veterinary Science 93(2):695-704.
  3. Kushibiki, S., Hodate, K Shingu, H, Obara, Y, Touno, E, Shinoda, M, and Y. Yokomizo. (2003). Metabolic and lactational responses during recombinant bovine tumor necrosis factor-α treatment in lactating cows. J. Dairy Sci. 86:819–827.
  4. Li, S, Khafipour, E, Krause, D.O, Kroeker, A, Rodriguez-Lecompte, J, Gozho, G.N. and J. C. Plaizier (2012). Effects of subacute ruminal acidosis challenges on fermentation and endotoxins in the rumen and hindgut of dairy cows, J. Dairy Sci. 95:294–303.
  5. Sanz-Fernandez, M.V, Daniel, J, Seymour, D.J, Kvidera, S.K, Bester, Z, Doelman, J. and J. Martín-Tereso (2020). Targeting the Hindgut to Improve Health and Performance in Cattle, Animals, 10: 1817.
  6. Watanabe, D.H.M, Doelman, J, Steele, M.A, Guan, L. and G. B. Penner (2020). Evaluating the effect of Ca-gluconate and Ca-butyrate on SCFA absorption and permeability of the gastrointestinal tract. J. Anim. Sci Vol. 97, Suppl. S3.
  7. Santos, A, Bergman, J.G.H.E, Manzano, J.A. and M. Hall (2023). Rumen protected calcium gluconate increases average daily gain of beef. Proceedings of the EAAP congress, Lyon, August 27-September 1, 562.
  8. Rossi, C.A.S, Grossi, S, van Kuijk, S and S. Vandoni (2024). Effect of the administration of a protected source of calcium gluconate on growth, feed efficiency, nutrient digestibility, and health in beef cattle. Proceedings of the ASAS, Calgary, July 21-25.
  9. Osman, Y., Koyun, E., Rowland, J., Lourenco, J., Baloyi, F.L., Fluharty, F., Pringle, T.D., Stewart, R.L., McCarthy, K., Griswold, K.E., and T.R. Callaway (2022). Impact of calcium gluconate feeding on intestinal microbial populations in a growing steer model. University of Georgia. Osman, Y., PhD Thesis.

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